Biopolym. Cell. 1997; 13(3):245-249.
Viruses and Cell
Permissivness of human embryo liver cells for HIV infection
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
5, Amosova Str., Kyiv, Ukraine, 03038
We have studied permissivness of embryonic liver cells to HIV-1 infection. The data obtained on primary cultivated cell populations from 25 aborted embryos have shown those liver stem cells could be regarded as a highly permissive for HIV-1 infection and reproduction. The titers of infectious virus and its p24 antigen produced in native cells were substantially higher than that in standard culture of T4-lymphoma cells MT-4. We have not found HIV-resistan1 samples of embryonic liver cells, nevertheless two isolations of cell samples revealed by low permissivness contrary to the majority of cases. We suggest that these primary cells could be used as alternative to established ones, when the use of immortalized transformed cell lines (MT-4 or any other) is not desirable. The hypothesis of blood cell's native resistance to HIV infection is also discussed.
 Riddell SR, Greenberg PD, Overell RW, Loughran TP, Gilbert MJ, Lupton SD, et al. Phase I Study of Cellular Adoptive Immunotherapy Using Genetically Modified CD8 + HIV-Specific T Cells for HIV Seropositive Patients Undergoing Allogeneic Bone Marrow Transplant. Fred Hutchinson Cancer Research Center and the University of Washington . Human Gene Therapy. 1992;3(3):319вЂ“38.
 Appelbaum FR, Sullivan KM, Buckner CD, Clift RA, Deeg HJ, Fefer A, Hill R, Mortimer J, Neiman PE, Sanders JE, et al. Treatment of malignant lymphoma in 100 patients with chemotherapy, total body irradiation, and marrow transplantation. J Clin Oncol. 1987;5(9):1340-7.
 Baltimore D. Gene therapy. Intracellular immunization. Nature. 1988;335(6189):395-6.
 Caiman A. Antisense strategies in cell and developmental biology. J Cell Sci. 1990; 97: 399-409.
 Yu M, Poeschla E, Wong-Staal F. Progress towards gene therapy for HIV infection. Gene Ther. 1994;1(1):13-26.
 Sczakiel G, Oelze L, Rittner K. Microinjections: a technique to study inhibition of HIV-l replication mediated by antisense RNA and parvovirus genes. Biotechnology applications of microinjection, microscopic imaging, and fluorescence. Eds P. H. Bach et aL-New York: Plenum press. 1993: 1-10.
 Yu M, Ojwang J, Yamada O, Hampel A, Rapapport J, Looney D, Wong-Staal F. A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1. Proc Natl Acad Sci U S A. 1993;90(13):6340-4.
 Hermonat PL, Muzyczka N. Use of adeno-associated virus as a mammalian DNA cloning vector: transduction of neomycin resistance into mammalian tissue culture cells. Proc Natl Acad Sci U S A. 1984;81(20):6466-70.
 Kukharenko O, Lukash L, Ivanska N et al. Construction of eukaryotic vector based on AAV, which makes the synthesis of RNA in the cell antysensovyh against HIV. The first national. Science. Pract. Conf. with HIV. AIDS: Abstracts. (Kyiv, 24-26 Jan 1995). Kyiv, 1995: 61-62.
 Grishchenko VI, Lobyntseva GS, Votyakova IA et al. Clinical use of hematopoietic cells cryopreserved human fetal liver. Method recommendation of the Ministry of Health of the UkrSSR. Kiev: RCNII, 1991; 9 p.
 Grishchenko VI, Lobyntseva GS, Votyakova IA, Shereshkov SI. Hematopoietic fetal liver cells (embryogenesis, cryopreservation and transplantation). Kiev: Naukova Dumka, 1988. 190 p.
 Sukhorada HM, Lukash LL, Podolskaya SV, Shved AD, Smykodub AI. The way of receiving and cultivating of human embryonic hepatocytes. Biopolym Cell. 1995; 11(1):92-4.
 Levy JA. HIV pathogenesis and long-term survival. AIDS. 1993;7(11):1401-10.
 Easterbrook PJ. Non-progression in HIV infection. AIDS. 1994;8(8):1179-82.
 Buchbinder SP, Katz MH, Hessol NA, O'Malley PM, Holmberg SD. Long-term HIV-1 infection without immunologic progression. AIDS. 1994;8(8):1123-8.
 Pantaleo G, Menzo S, Vaccarezza M, Graziosi C, Cohen OJ, Demarest JF, Montefiori D, Orenstein JM, Fox C, Schrager LK, et al. Studies in subjects with long-term nonprogressive human immunodeficiency virus infection. N Engl J Med. 1995;332(4):209-16.
 Learmont J, Tindall B, Evans L, Cunningham A, Cunningham P, Wells J, Penny R, Kaldor J, Cooper DA. Long-term symptomless HIV-1 infection in recipients of blood products from a single donor. Lancet. 1992;340(8824):863-7.
 Cao Y, Qin L, Zhang L, Safrit J, Ho DD. Virologic and immunologic characterization of long-term survivors of human immunodeficiency virus type 1 infection. N Engl J Med. 1995;332(4):201-8.
 Baltimore D. Lessons from people with nonprogressive HIV infection. N Engl J Med. 1995;332(4):259-60.
 Kirchhoff F, Greenough TC, Brettler DB, Sullivan JL, Desrosiers RC. Brief report: absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection. N Engl J Med. 1995;332(4):228-32.
 Samson M, Libert F, Doranz BJ, Rucker J, Liesnard C, Farber CM, Saragosti S, Lapoumeroulie C, Cognaux J, Forceille C, Muyldermans G, Verhofstede C, Burtonboy G, Georges M, Imai T, Rana S, Yi Y, Smyth RJ, Collman RG, Doms RW, Vassart G, Parmentier M. Resistance to HIV-1 infection in caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene. Nature. 1996;382(6593):722-5.
 Kukcharenko AP, Kogut GI, Shved AD. The scientific and practical use of umbilical cord blood hematopoietic cells. Biopolym Cell. 1995; 11(2):5-14.