Biopolym. Cell. 1997; 13(2):121-126.
http://dx.doi.org/10.7124/bc.000473
Structure and Function of Biopolymers
Cloning of cDNA encoding C-terminal part of mammalian tyrosyl-tRNA synthetase using of PCR-amplified radioactive probe
1Levanets O. V., 1Naidenov V. G., 1Woodmaska M. I., 1Matsuka G. H., 1Kornelyuk A. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Radioactive probe of high activity (3.5·109 cpm/μg) specific to mammalian tyrosyl-tRNA synthetase has been synthesized by polymerase chain reaction. Bovine liver cDNA-library has been screened and specific clone has been detected. Sequencing of cDNA insert shows that the done encodes C-terminal part of bovine tyrosyl-tRNA synthetase. C-terminal non-canalytic domain of mammalian tyrosyl-tRNA synthetase shares homology of amino acid sequence with a novel cytokine – endothelial-monocyte activating polypeptide II.
Full text: (PDF, in Russian)

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