Biopolym. Cell. 1997; 13(1):60-62.
http://dx.doi.org/10.7124/bc.000468
Cell Biology
Genetic transformation of Digitalis purpurea by Agrobacterium tumefaciens
1Pogrebnyak N. Ya., 1Shisha L. N., 1Storozhenko S. V., 1Yuzefovich L. I., 2Dittrich B., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
  2. Martin-Luther-Universitat Halle-Wittenberg
    Halle (Saale) Germany

Abstract

Agrobacterium-mediated method of transformation of D. purpurea has been developed. Transgenic geneticin-resistant D. purpurea lines have been obtained. Transgenic nature of these lines have been confirmed by PCR.
Full text: (PDF, in Russian)

References

[1] Scott RJ, Draper J. Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants. Plant Mol Biol. 1987;8(3):265-74.
[2] An G. High efficiency transformation of cultured tobacco cells. Plant Physiol. 1985;79(2):568-70.
[3] Pollock K, Barfield DG, Robinson SJ, Shields R. Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens. Plant Cell Rep. 1985;4(4):202-5.
[4] Wurtele ES, Bulka K. A simple, efficient method for the Agrobacterium-mediated transformation of carrot callus cells. Plant Sci. 1989;61(2):253–62.
[5] D’Halluin K, Bossut M, Bonne E, Mazur B, Leemans J, Botterman J. Transformation of sugarbeet (Beta vulgaris L.) and evaluation of herbicide resistance in transgenic plants. Bio/Technology. 1992;10(3):309–14.
[6] Zhang H, Somerville CR. Transfer of the maize transposable element MU1 into Arabidopsis thaliana. Plant Science. 1987;48(3):165–73.
[7] Diettrich B, Steup C, Neumann D, Scheibner H, Reinbothe C, Luckner M. Morphogenetic Capacity of Cell Strains Derived from Filament, Leaf and Root Explants of Digitalis lanata. J Plant Physiol. 1986;124(5):441–53.
[8] Storozhenko SV. Analysis of transgenic plants using polymerase chain reaction: the pair of universal primers for amplification of 35S promoter sequence. Biopolym Cell. 1994;10(1):72-5.
[9] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980;8(19):4321-5.