Biopolym. Cell. 1996; 12(6):50-56.
Changes in fish blood under the effect of chemical pollution studied by low-temperature 1H-NMR
1Goryunov A. S., 1Sukhanova G. A., 1Borisova A. G., 1Sidorov V. S.
- Institute of Biology, Karelian Research center
11, Pushkinskaya Str., Petrozavodsk, Karelia, Russian Federation, 185610
Abstract
A n approach to comparative studies of the changes in blood serum under the effect of internal and external environmental non-specific factors is developed on the basis of low-temperature 1H-NMR. Under investigation were two groups of breams from the zones differing in the degree of pollution with polychlorated biphenyls, or PCB. The hydrationof protein in the sera offish from heavily polluted zones was found to be significantly greater than that of weakly polluted zones. It is shown to be due to the decrease of the total protein concentration, leading to the change in the ratio between ions and protein components of serum. The assumed consequence is the difference in the type of interaction of ions with protein in two groups of bream sera as well as the influence of this interaction on the hvdration and the aeerezation of macromolecules.
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References
[1]
Vashman AA Pronin NS. Nuclear magnetic relaxation spectroscopy. M .: Energoizdat, 1986. 232 p.
[2]
Raeymaekers HH, Borghys D, Eisendrath H. Determinants of water proton T1 in blood serum. Magn Reson Med. 1988;6(2):212-6.
[3]
Zefirova TP, Glebov AN, Gur'ev EN, Mavliautdinov RS, Tarasov OIu. [Nuclear magnetic relaxation of aqueous solutions of proteins, plasma, erythrocytes, and blood]. Biull Eksp Biol Med. 1991;112(10):378-81.
[4]
Ba?keev RF, Kha?rullina VR, Ziatdinov KM, Mazitova FM, Mavliutova RI. [The measurement of NMR-1H relaxation time of blood serum as a method of laboratory diagnosis]. Lab Delo. 1990;(12):17-9.
[5]
Zefirova TP, Glebov AN. [Nuclear magnetic relaxation in biochemical analysis of plasma and blood serum]. Biull Eksp Biol Med. 1992;114(7):38-9.
[6]
Beall PT, Medina D, Hazlewood CF. The “Systemic Effect” of Elevated Tissue and Serum Relaxation Times for Water in Animals and Humans with Cancers. NMR in Medicine. Springer. 1981;39–57.
[7]
Kuntz ID Jr, Brassfield TS, Law GD, Purcell GV. Hydration of macromolecules. Science. 1969;163(3873):1329-31.
[8]
Hays DL, Fennema O. Methodology for determining unfreezable water in protein suspensions by low-temperature NMR. Arch Biochem Biophys. 1982;213(1):1-6.
[9]
Kalb VF Jr, Bernlohr RW. A new spectrophotometric assay for protein in cell extracts. Anal Biochem. 1977;82(2):362-71.
[10]
Kudryavtsev AA, Kudryavtseva LA, Privolnaya TI Hematology animals and fish. M.: Kolos, 1969. 320 p.
[11]
Kuntz ID Jr, Kauzmann W. Hydration of proteins and polypeptides. Adv Protein Chem. 1974;28:239-345.
[12]
Rupley JA, Careri G. Protein hydration and function. Adv Protein Chem. 1991;41:37-172. Review.
[13]
Cavatorta F, Fontana MP, Velci A. Raman spectroscopy of protein–water interactions in aqueous solutions. J Chem Phys. 1976;65(9):3635-40.
[14]
Gasan AI, Kashpur VA, Maleev VIa. [Thermal restructuring and hydration of serum albumin]. Biofizika. 1994;39(4):588-93.
[15]
Maksimenko AV, Morozkin AD, Torchilin VP. [Salt ion interaction with the protein molecule: the facts and the concept]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1986;(12):5-18.
[16]
Kuntz ID Jr, Brassfield TS. Hydration of macromolecules. II. Effects of urea on protein hydration. Arch Biochem Biophys. 1971;142(2):660-4.
