Biopolym. Cell. 1996; 12(2):74-83.
http://dx.doi.org/10.7124/bc.000425
Viruses and Cell
Aromatic thiosemicarbazones, their antiviral action and interferon. 1. The decreasing of adenovirus type 1 resistance against interferon by methisazone in vitro
1Patskovsky Yu. V., 1Negrebetskaya E. N., 2Chernomaz A. A., 1Voloshchuk T. P., 1Rubashevsky E. L., 1Kitam O. E., 1Tereshchenko M. I., 2Nosach L. N., 1Potopalsky A. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

The mechanism of N-mvthyl-izatin-thiocarbazone (methisazone, MelBT) antiviral activity has been studied on Ad 1-infected HEp2 and HeLa cells. MelBT did not induce interferon and did not directly inhibit viraland cell translation. The adenoviral infection was not affected by recombinani human interferon a2 (rlFN). MelBT showed antiviral effect in Adl-infected HEp2 or HeLa cells when rlFN had added to HeLa cells or in the period of interferon induction during virus infection (in HEp2 cells). In the presence of this compound, the ElA transcription was unchanged in infected cells as compared to untreated control, while early transcription was decreased, the beginning of viral replication being retarded. Futhermore, the VA1 RNA synthesis was also greatly suppressed. These effects were independent on interferon treatment and disappeared when MelBT had been added during the late phase of virus growth cycle. ctually, MelBT can induce the delay of VA1 RNA transcription promoting interferon antiviral effect.
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