Biopolym. Cell. 1996; 12(2):68-73.
http://dx.doi.org/10.7124/bc.000424
Genome and Its Regulation
Approaches to gene identification of Campylobacteria genus bacteria by polymerase chain reaction with universal primers
1Kirik D. L., 2Burianovsky L. N., 1Sliablovskaya E. A., 1Pinchuc I. V., 1Krolevetskaya N. M., 1Kikot V. I.
  1. Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Preliminary studies on development of gene identification method of Campylobacteria genus bacteria on the base of polymerase chain reaction (PCR) with using universialoligonucleotide primers – REP (repetitive extragenic palindromic sequences). For all strains of Campylobacter jejuni PCR-product was peculiar 600 pairs ofnucleotides. Five group ofPCR-serovars in strains C. jejuni, one serovar (Lio 32) differed on minoric fragments. It can be of importance for difinition of infection source while epidemic analysis.
Full text: (PDF, in Russian)

References

[1] Fox JG, Taylor NS, Penner JL, Shames B, Gurgis RV, Tomson FN. Investigation of zoonotically acquired Campylobacter jejuni enteritis with serotyping and restriction endonuclease DNA analysis. J Clin Microbiol. 1989;27(11):2423-5.
[2] Nachamkin I, Bohachick K, Patton CM. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol. 1993;31(6):1531-6.
[3] Giesendorf BA, van Belkum A, Koeken A, Stegeman H, Henkens MH, van der Plas J, Goossens H, Niesters HG, Quint WG. Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol. 1993;31(6):1541-6.
[4] Sultanov GV, Cherkassky BL, Navashin SM et al. A new medium for the diagnosis of campylobacteriosis. Especially dangerous infections in the Caucasus: Proc. sixth boundary scientific. Conf. Stavropol, 1987: 276-8.
[5] Instructions for clinical and laboratory diagnosis of campylobacteriosis. M., 1989. 25 p.
[6] Lior H, Woodward DL, Edgar JA, Laroche LJ, Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982;15(5):761-8.
[7] Higgins CF, Ames GF, Barnes WM, Clement JM, Hofnung M. A novel intercistronic regulatory element of prokaryotic operons. Nature. 1982;298(5876):760-2.
[8] Versalovic J, Koeuth T, Lupski JR. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991;19(24):6823-31.
[9] Ausubel FM. Current protocols in molecular biology. New York: Wiley, 1987: 241.
[10] Chen WP, Kuo TT. A simple and rapid method for the preparation of gram-negative bacterial genomic DNA. Nucleic Acids Res. 1993;21(9):2260.