Analysis of DNA from strains of malaria parasites sensitive and resistant to chloroquine by restriction endonuclease fingerprinting

Pankova, T. G.; Igonina, T. M.; Klimova, E. R.; Mayer, T. V.

doi:10.7124/bc.0003DF

Biopolym. Cell. 1995; 11(2):46-51.

http://dx.doi.org/10.7124/bc.0003DF

Analysis of DNA from strains of malaria parasites sensitive and resistant to chloroquine by restriction endonuclease fingerprinting

1Pankova T. G., 1Igonina T. M., 1Klimova E. R., 1Mayer T. V.

Institute of Cytology and Genetics, Siberian Branch of the RAS
10, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090

Abstract

DNAs from various Plasmodium berghei strains with different chloroquine (Chi) sensitivities were analysed by agarose gel electrophoresis following digestion with restriction endonucleases EcoRI, Hindlll and BamHI. The DNA patterns for the two Chl-resistant strains – LNK-65 strain with 2–3 fold increased Chl-resistance and LNK ChlR line with 7–10 fold increased, Chl-resistance (derived from the parent LNK-65 strain by stepwise selection in Chi) show similarity, but some additional bands of DNA digests are observed for LNK ChlR strain compared to the parent LNK strain. DNAs of malarial parasites from the sensitive N strain and from the Chl-resistant strains (LNK-65 and LNK ChlR) yield recognizably distinct patterns, so the resistant strains are suggested to belong to another Plasmodium species.

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