Biopolym. Cell. 1995; 11(1):45-49.
Identification of the conserved structural elements in the ribosomal protein L10 which are essential for the autogenous regulation of gene expression in the rplJL operon of Enterobacteria
1Tereshchenko F. A., 1Paton E. B.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

We analyzed the structural elements of the ribosomal protein L10 which are essential for interaction with the mRNA target sites and therefore for execution of the autogenous control of expression of the rplJL genes in Enterobacteria. The following structural elements of the L10 protein might be essential the feedback regulatory for L10-mRNA interaction: the conserved-kNTll-motif, the -Eif-motif and the alpha-beta-alpha (98–111) domain.

References

[1] Paton EB, Woodmaska MI, Kroupskaya IV, Zhyvoloup AN, Matsuka GKh. Evidence for the ability of L10 ribosomal proteins of Salmonella typhimurium and Klebsiella pneumoniae to regulate rplJL gene expression in Escherichia coli. FEBS Lett. 1990;265(1-2):129-32.
[2] Zhyvoloup A. N., Kiritchenko I. V., Paton E. B. Ribosomal protein L10 of Escherichia coli can regulate Salmonella typhimurium rplJL gene expression. Biopolym. Cell. 1992; 8(3):37-39.
[3] Zhyvoloup A. N., Paton E. B. Ribosomal protein L10 of Escherichia coli is regulating gene expression in the rplJL operon of Citrobacter freundii. Biopolym. Cell. 1993; 9(6):90-9.
[4] Zhyvoloup A. N., Kroupskaya I. V., Lyaskovsky T. M., Paton E. B. Comparison of nucleotide sequences of the rplJL leader in Enterobacteria. Biopolym. Cell. 1994; 10(1):37-40.
[5] Egebjerg J, Douthwaite SR, Liljas A, Garrett RA. Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli. J Mol Biol. 1990;213(2):275-88.
[6] Vagis A. G., Bratus' A. V., Vasilenko V. I. The implementation of the expert system based on the logical simulation. Kiev : Izd-vo In-ta kibernetiki im. V. M. Glushkova, 1987. 16 s.
[7] Nagai K. RNA-protein interactions. Curr Opin Struct Biol. 1992; 2(1): 131-7.
[8] Lazinski D, Grzadzielska E, Das A. Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif. Cell. 1989;59(1):207-18.
[9] Climie SC, Friesen JD. In vivo and in vitro structural analysis of the rplJ mRNA leader of Escherichia coli. Protection by bound L10-L7/L12. J Biol Chem. 1988;263(29):15166-75.
[10] Liao D, Dennis PP. The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima. J Biol Chem. 1992;267(32):22787-97.
[11] Schmidt J, Bubunenko M, Subramanian AR. A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. J Biol Chem. 1993;268(36):27447-57.
[12] Pettersson I. Studies on the RNA and protein binding sites of the E. coli ribosomal protein L10. Nucleic Acids Res. 1979;6(7):2637-46.
[13] Paton EB, Krupskaia IV, Zhivolup AN. Determination of a minimal segment of E. coli ribosomal protein L10 retaining its regulatory function. Dokl Akad Nauk SSSR. 1989;309(2):493-6.