Biopolym. Cell. 1994; 10(1):47-52.
Study of 2', 5'-oligoadenylates analogues stability to the action of phosphodiesterases
1Kozlov A. V., 1Tkachuk Z. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A wide variety of 2', 5'-oligoadenylates stability to the action of phosphodiesterases was studied by capillary electrophoresis and HPLC. The original 2', 5'-ApApA «core» was shown to be considerably hydrolyzed and adenosine, AMP and 2', 5'ApA were formed. Sugar modification by 3' and 2' position results in the stability increase, the degree of stability depending on the type of modification. The mechanism of this stability is probably bound with the modified analogues affinity to phosphodiesterase defect.


[1] Torrence PF, Lesiak K, Imai J, Johnston MI, Sawai H. 2?,5?-Oligoadenylates: Their Role in Interferon Action and Their Potential as Chemotherapeutic Agents. Nucleosides, Nusleotides and their Biological Applications. 1983;67–115.
[2] Marti J, Roux D, Favero J, Dornand J, Cailla H. Characterization of a Novel 2?,5? Oligoadenylate in Stimulated Lymphocytes. Nucleosides Nucleotides. 1988;7(4):479–95.
[3] Hearl WG, Johnston MI. Accumulation of 2',5'-oligoadenylates in encephalomyocarditis virus-infected mice. J Virol. 1987;61(5):1586-92.
[4] Bosworth BT, MacLachlan NJ, Johnston MI. Induction of the 2-5A system by interferon and transmissible gastroenteritis virus. J Interferon Res. 1989;9(6):731-9.
[5] Floyd-Smith G. (2'-5')An-dependent endoribonuclease: enzyme levels are regulated by IFN beta, IFN gamma, and cell culture conditions. J Cell Biochem. 1988;38(1):13-21.
[6] M?ller WE, Weiler BE, Charubala R, Pfleiderer W, Leserman L, Sobol RW, Suhadolnik RJ, Schr?der HC. Cordycepin analogues of 2',5'-oligoadenylate inhibit human immunodeficiency virus infection via inhibition of reverse transcriptase. Biochemistry. 1991;30(8):2027-33.
[7] White JC, Williams RW, Johnston MI. Raman spectroscopy of interferon-induced 2',5'-linked oligoadenylates. Biochemistry. 1987;26(24):7737-44.
[8] Tkachuk ZYu, Tkachuk LV, Kozlov AV, Kvasyuk EI, Zaitseva GV, Kalinichenko EN, Mikhailopulo IA, Matsuka GKh. Effect of (2'-5')oligoadenylates on the hydrolysis of the S1 nuclease and DNA l complex in vitro. Doklady Akad Nauk Ukr SSR. Ser B. 1988; (12):62-6.
[9] Bayard B, Bisbal O, Silhol M et al. Increased stability and antiviral activity of 2'-O-phosphoglyceryl derivatives of (2'-5')oligo(adenylate). Roles of phosphodiesterases and phosphatases in (2'-5')oligo(adenylate) catabolism. Eur J Biochem. 1984. 142(2):291-8.
[10] Correia M, Jamoulle JC, Rentier B. Liposome-mediated cellular internalization of (pA3). Arch Int Physiol Biochem. 1986; 94(1):B10.
[11] Leonetti J-P, Degols G, Milhaud P, Gagnor C, Lemaitre M, Lebleu B. Antiviral activity of antisense oligonucleotides linked to poly(L-lysine):targets on genomic RNA and/or mRNA of Vesicular Stomatitis Virus. Nucleosides Nucleotides Nucleic Acids. 1989;8(5):825–8.
[12] Tominaga A, Saito S, Kohno S, Sakurai K, Hayakawa Y, Noyori R. Antiviral effects of 2',5'oligoadenylates (2-5As), and related compounds. Microbiol Immunol. 1990;34(9):737-47.
[13] Torrence PF, Imai J, Lesiak K, Jamoulle J-C, Sawai H, Warinnier J, et al. Strategies in the Design of Oligonucleotides as Potential Antiviral Agents. Targets for the Design of Antiviral Agents. 1984;259–85.