Study of 2\', 5\'-oligoadenylates analogues stability to the action of phosphodiesterases

Kozlov, A. V.; Tkachuk, Z. Yu.

doi:10.7124/bc.000390

Biopolym. Cell. 1994; 10(1):47-52.

http://dx.doi.org/10.7124/bc.000390

Study of 2', 5'-oligoadenylates analogues stability to the action of phosphodiesterases

1Kozlov A. V., 1Tkachuk Z. Yu.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A wide variety of 2', 5'-oligoadenylates stability to the action of phosphodiesterases was studied by capillary electrophoresis and HPLC. The original 2', 5'-ApApA «core» was shown to be considerably hydrolyzed and adenosine, AMP and 2', 5'ApA were formed. Sugar modification by 3' and 2' position results in the stability increase, the degree of stability depending on the type of modification. The mechanism of this stability is probably bound with the modified analogues affinity to phosphodiesterase defect.

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