Biopolym. Cell. 1985; 1(6):318-322.
http://dx.doi.org/10.7124/bc.000194
Short Communications
Interaction of the tRNAPhe-C-C-A(3'NH)-Phe with a small subunit of Escherichia coli
1Dorokhov D. B., 1Shwarts V. S., 2Semenkov Yu. P.
  1. The Department of Plant Genetics, Academy of Sciences of the Moldavian SSR
    Kishinev, USSR
  2. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR

Abstract

The thermodynamic parameters of binding of tRNAPhe-C-C-A(3'NH)Phe (a stable analogue of aminoacyl-tRNA) to SOS ribosomal subunit are studied. In the presence of template 30S subunit binds 2 molecules of tRNAphe-C-C-A(3'NH)-Phe to the acceptor (A) and peptidyl (P) sites. The affinity constants of the stable analogue of aminoacyl-tRNA for tRNA-binding sites of the small ribosomal subunit are measured: KaP=(3.5±0.35). 108 M–1 and KaA~3-107 M–1. The results obtained indicate the great similarity in binding characteristics between Phe-tRNAphe and tRNAPhe-C—C—A(3'NH)-Phe. It permits applying tRNAPhe-C-C-A(3'NH)-Phe as a very convenient model to study interaction between aminoacyl-tRNA and SOS subunit.
Full text: (PDF, in Russian)

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