Biopolym. Cell. 1985; 1(5):247-253.
Reviews
E. coli DNA polymerase I:
primer-template-dependent enzyme inactivation
by imidazolides of deoxynucleoside-5'-triphosphates
- Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
Novosibirsk, USSR
Abstract
Interaction of E. coli DNA-polymerase I with imidazolides of dATP, dCTP, dGTP and dTTP is investigated in the presence and the absence of different primer-template complexes. It is found that the enzyme can be inactivated only in the presence of a primer and a template being complementary to dNTP analogue. From the data obtained it may be supposed that the orientation of analogue polyphosphate chain changes due to the analogue-template complex formation. Such a transformation results in the close proximity of the terminal phosphate of the analogue to nucleophilic groups of the active enzyme site and in their subsequent phosphorylation.
Full text: (PDF, in Russian)
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