Catalytic activity of aspartate aminotransferase microcrystals
DOI:
https://doi.org/10.7124/bc.00019EAbstract
The enzymic activity of crystalline cytosolic aspartate aminotransferase (L-aspartate: aminotransferase 2-oxoglutarate, EC 2.6.1.1) was determined in suspensions of micro-crystals in 50 % ammonium sulphate and 20 % (wt/vol) polyethylene glycol. The crystals (1-2 μm wide) were small enough to preclude diffusional rate limitation. The catalytic activity of the crystalline enzyme is 32 % and 40 % of the enzyme activity in solution, respectively.References
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