Formamide and glycerol in sequencing of GC-rich DNA

Authors

  • S. M. Kvasha Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680; Center for Genomics and Bioinformatics, Karolinska Institutet SE-171 77 Stockholm, Sweden Author
  • E. R. Zabarovsky Center for Genomics and Bioinformatics, Karolinska Institutet SE-171 77 Stockholm, Sweden; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute Nobels vag 5 171 65 Solna, Sweden; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences 32, Vavilova Str., Moscow, Russian Federation, 119991 Author
  • V. I. Kashuba Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680; Center for Genomics and Bioinformatics, Karolinska Institutet SE-171 77 Stockholm, Sweden; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute Nobels vag 5 171 65 Solna, Sweden Author
  • A. V. Rynditch Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0005B5

Abstract

Formamide and glycerol are known to increase the sequencing efficiency. We compared the ability of different concentration of these co-solvents to improve the sequencing results of GC-rich template. Herein, we report that addition of 5 % formamide and 7.5–10 % glycerol is the most effective for sequencing GC-rich templates.

References

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Zhang W, Reading C, Deisseroth AB. Improved PCR sequencing with formamide. Trends Genet. 1992;8(10):332.

Zhang W, Hu GY, Deisseroth A. Improvement of PCR sequencing by formamide. Nucleic Acids Res. 1991;19(23):6649.

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Published

2001-05-20

Issue

Vol. 17 No. 3 (2001)

Section

Short Communications