Biopolym. Cell. 1993; 9(4):63-104.
http://dx.doi.org/10.7124/bc.000368
Генно-інженерна біотехнологія
Генна терапія масових патологій
1Кордюм В. А., 1Фролькіс В. В., 1Лукаш Л. Л., 1Шульженко В. Н., 1Шпильова С. П., 1Костецький І. Є., 1Тіток Т. Г., 1Варзанова І. С., 1Ліхачова Л. І., 1Іродов Д. М.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680

Abstract

Описано комплекс досліджень, яким дозволяє пройти шлях від ідеї до експериментального вирішення принципової можливості генної терапії масових патологій, на прикладі інсулінзалежного цукрового діабету та атеросклерозу. Зроблено добір та аналіз регуляторних елементів, які дозволяють здійснювати експресію екзогенних генів незалежно від стану загальної клітинної регуляції. На клітинах різних тканин та організмів, а також in vivo показано експресію генетичного матеріалу, що вводиться (як модельного гена бактеріальної β-галактозидази, так і генів інсуліну та аполіпопротеїну високої щільності Al, які мають відношення до вищезгаданих патологїй). Відзначено неправомірність екстраполяції результатів, отриманих в культурі клітин, на тварин; показано неоднозначність експресії рекомбінантних молекул залежно від генного оточення і типу клітин реципієнтів. Як в культурі клітин, так і в організмові виявлено індивідуально-клітинну гетерогенність кількісних характеристик експресі: введеного ззовні гена. Крім того, відзначено індивідуально-організмову та вікову гетерогенність експресії екзогенної інформації. Зроблено висновок про необхідність індивідуалізації генної терапії масових патологів.
Повний текст: (PDF, англійською)

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