Biopolym. Cell. 1988; 4(1):20-27.
Структура та функції біополімерів
Зміна констант ДНК-білкового зв’язування за допомогою комплексу хроматограф «Милихром»?ЕОМ
1Каламбет Ю. А., 1Бурова Е. І., 1Жучков А. А., 1Кнорре В. Л., 1Александров А. А.
  1. Інститут молекулярної генетики АН СРСР
    Москва, СРСР

Abstract

Для определения констант ДНК-белкового связывания использован метод гель-хроматографии. В качестве регистрирующего прибора применен комплекс, состоящий из многоволнового сканирующего хроматографа «Милихром» и ЭВМ «Искра-226». Программное обеспечение, разработанное для этого комплекса, позволяет в любой момент времени определять концентрации ДНК и белка. Получаемая информация о зависимости концентраций ДНК и белка от времени используется для определения констант ДНК-белкового связывания. Метод применен к исследованию взаимодействия РНК-полимеразы Е. соli с рядом плазмидных ДНК и четырьмя одноните-выми олигодезоксирибонуклеотидами — фрагментами промоторов Е. coli

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