Biopolym. Cell. 2023; 39(2):110-130.
Геноміка, транскриптоміка та протеоміка
Передбачення та аналіз стрес-індукованих транскрипційних факторів ICE у Deschampsia antarctica
1Бублик О. М., 1Андрєєв І. О., 1Кунах В. А.
- Інститут молекулярної біології і генетики НАН України
Вул. Академіка Заболотного, 150, Київ, Україна, 03143
Abstract
Мета. Дослідження молекулярно-генетичних аспектів стресостійкості рослини-екстремофіла D. antarctica на прикладі аналізу транскрипційних факторів ICE, залучених до відповіді на холодовий стрес, регуляції розвитку продихів і часу цвітіння. Методи. Гени DaICE збирали із послідовностей, гомологічних до генів-ортологів, з бази даних SRA. Передбачені гени та білки аналізували біоінформатичними методами. Генну експресію аналізували з використанням даних секвенування РНК. Результати. Знайдено два варіанти гена DaICE1: типовий, який кодує повнорозмірний білок, та варіант із великою делецією, що призвела до втрати N-кінця та частини bHLH-домена у білкового продукта. Для гена DaICE2 знайдено два близькі варіанти з чотирма однонуклеотидними поліморфізмами, три з яких є синонімічними, в кодувальній ділянці та більш відмінними промоторними послідовностями. Досліджено організацію промоторів та інтрон-екзонну структуру генів. Для передбачених ТФ визначено фізико-хімічні властивості, показано локалізацію в ядрі, можливість посттрансляційних модифікацій, проведено філогенетичний аналіз, пошук консервативних мотивів, передбачено тривимірну структуру. Виявлено експресію генів у більшості або всіх тканинах та показано підвищену експресію варіантів DaICE1 в рослинах з природних умов, порівняно з лабораторними. Висновки. DaICE1 та DaICE2 є кандидатами на роль регуляторів експресії генів CBF під час холодового стресу у D. antarctica.
Keywords: антарктичні рослини, транскрипційні фактори, передбачення in silico, абіотичний стрес, стійкість
Повний текст: (PDF, англійською)
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