Biopolym. Cell. 1987; 3(1):3-17.
Огляди
Антисенсові РНК: новий механізм регуляції генної експресії
1Швед А. Д., 1Золотухін С. Б., 1Мацука Г. Х.
  1. Інститут молекулярної біології і генетики АН УСРС
    Київ, СРСР

Abstract

Огляд присвячено стану питання про нещодавно відкритий новий механізм регулювання генної експресії, заснований на РНК:РНК-взаємодії. Наведено відомості про виявлені в природі приклади регуляторної функції антисенсових РНК, про загальні принципи конструювання штучних антисенсових регуляторних систем, а також представлено аналіз робіт, присвячених експериментальній перевірці активності різних антнсенсових РНК-транскриптів у репресії окремих генів.

References

[1] Simons RW, Kleckner N. Translational control of IS10 transposition. Cell. 1983;34(2):683-91.
[2] Cesareni G, Banner DW. Regulation of plasmid copy number by complementary RNAs. Trends Biochem Sci. 1985; 10(8):303-6.
[3] Tomizawa J, Itoh T, Selzer G, Som T. Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA. Proc Natl Acad Sci U S A. 1981;78(3):1421-5.
[4] Tomizawa J. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible. Cell. 1985;40(3):527-35.
[5] Tomizawa J, Som T. Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein. Cell. 1984;38(3):871-8.
[6] Light J, Molin S. The sites of action of the two copy number control functions of plasmid R1. Mol Gen Genet. 1982;187(3):486-93.
[7] Light J, Molin S. Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule. EMBO J. 1983;2(1):93-8.
[8] Womble DD, Dong X, Wu RP, Luckow VA, Martinez AF, Rownd RH. IncFII plasmid incompatibility product and its target are both RNA transcripts. J Bacteriol. 1984;160(1):28-35.
[9] Kumar CC, Novick RP. Plasmid pT181 replication is regulated by two countertranscripts. Proc Natl Acad Sci U S A. 1985;82(3):638-42.
[10] Hoopes BC, McClure WR. A cII-dependent promoter is located within the Q gene of bacteriophage lambda. Proc Natl Acad Sci U S A. 1985;82(10):3134-8.
[11] Rezaian MA, Symons RH. Anti-sense regions in satellite RNA of cucumber mosaic virus form stable complexes with the viral coat protein gene. Nucleic Acids Res. 1986;14(8):3229-39.
[12] Spena A, Krause E, Dobberstein B. Translation efficiency of zein mRNA is reduced by hybrid formation between the 5'- and 3'-untranslated region. EMBO J. 1985;4(9):2153-8.
[13] Izant JG, Weintraub H. Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis. Cell. 1984;36(4):1007-15.
[14] Izant JG, Weintraub H. Constitutive and conditional suppression of exogenous and endogenous genes by anti-sense RNA. Science. 1985;229(4711):345-52.
[15] McGarry TJ, Lindquist S. Inhibition of heat shock protein synthesis by heat-inducible antisense RNA. Proc Natl Acad Sci U S A. 1986;83(2):399-403.
[16] Kim SK, Wold BJ. Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA. Cell. 1985;42(1):129-38.
[17] Harland R, Weintraub H. Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA. J Cell Biol. 1985;101(3):1094-9.
[18] Melton DA. Injected anti-sense RNAs specifically block messenger RNA translation in vivo. Proc Natl Acad Sci U S A. 1985;82(1):144-8.
[19] Preiss A, Rosenberg UB, Kienlin A, Seifert E, J?ckle H. Molecular genetics of Kr?ppel, a gene required for segmentation of the Drosophila embryo. Nature. 1985 Jan 3-9;313(5997):27-32.
[20] Rosenberg UB, Preiss A, Seifert E, J?ckle H, Knipple DC. Production of phenocopies by Kr?ppel antisense RNA injection into Drosophila embryos. Nature. 1985 Feb 21-27;313(6004):703-6.
[21] Crowley TE, Nellen W, Gomer RH, Firtel RA. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium. Cell. 1985;43(3 Pt 2):633-41.
[22] Mizuno T, Wurtzel ET, Inouye M. Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product. J Biol Chem. 1982;257(22):13692-8.
[23] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S. Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res. 1982;10(21):6957-68.
[24] Mizuno T, Chou MY, Inouye M. A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene. J Biol Chem. 1983;258(11):6932-40.
[25] Mizuno T., Chou M.-J., Inouye M. Regulation of gene expression by a small RNA transcript (micRNA) in Escherichia coli K-12. Proc. Jap. Acad. Sci. 1983; 59(10):335-8.
[26] Mizuno T, Chou MY, Inouye M. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA). Proc Natl Acad Sci U S A. 1984;81(7):1966-70.
[27] Coleman J, Green PJ, Inouye M. The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell. 1984;37(2):429-36.
[28] Coleman J, Hirashima A, Inokuchi Y, Green PJ, Inouye M. A novel immune system against bacteriophage infection using complementary RNA (micRNA). Nature. 1985 Jun 13-19;315(6020):601-3.
[29] Farnham PJ, Abrams JM, Schimke RT. Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene. Proc Natl Acad Sci U S A. 1985;82(12):3978-82.
[30] Pestka S, Daugherty BL, Jung V, Hotta K, Pestka RK. Anti-mRNA: specific inhibition of translation of single mRNA molecules. Proc Natl Acad Sci U S A. 1984;81(23):7525-8.
[31] Ellison MJ, Kelleher RJ 3rd, Rich A. Thermal regulation of beta-galactosidase synthesis using anti-sense RNA directed against the coding portion of the mRNA. J Biol Chem. 1985;260(16):9085-7.
[32] Rubenstein JL, Nicolas JF, Jacob F. Nonsense RNA: a tool for specifically inhibiting the expression of a gene in vivo. C R Acad Sci III. 1984;299(8):271-4.