Biopolym. Cell. 2010; 26(5):351-359.
Огляди
Конформаційна рухливість нуклеосом в експериментах з індивідуальними хроматиновими фібрилами
1Сиволоб А. В.
- Київський національний університет імені Тараса Шевченка
вул. Володимирська 64, Київ, Україна, 01601
Abstract
Дослідження нуклеосомної організації хроматину відіграє все більшу роль у розумінні механізмів регуляції генетичної активності. У представленому огляді описано результати вивчення конформаційної рухливості нуклеосом, отримані в експериментах з магнітним пінцетом – приладом, за допомогою якого можна індукувати торсійні деформації в олігонуклеосомних фібрилах, реконструйованих на індивідуальних молекулах ДНК. Такий підхід дозволяє виявити нову структурну форму нуклеосоми – реверсому, у складі якої ДНК формує праву суперспіраль на поверхні перебудованого октамеру гістонів. Обговорюються молекулярні механізми та біологічне значення структурної рухливості нуклеосом.
Keywords: нуклеосома, надспіралізація ДНК, хроматинова фібрила, конформаційна рухливість
Повний текст: (PDF, російською) (PDF, англійською)
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