Biopolym. Cell. 1990; 6(6):100-105.
Short Communications
Structural and functional characters of plasmid-like mitochondrial maize DNA
1Martynov S. A., 1Bukh I. G., 1Malyuta S. S.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Hybrid plasmids of two types containing S1 and S2-sequences of mitochondrial maize var. BUP 44 MC are obtained. The first type contains fragments, and the second one – the full sequences with the inverted repeated sequences in the centre of molecules. Recombinant plasmids of the second type have been shown not to be ARS-elements for yeast cells. While introducing hybrid plasmid into intact maize plants the low-weight fractions are found in mitochondrial DNA.


[1] Dixon LK, Leaver CJ, Brettell RI, Gengenbach BG. Mitochondrial sensitivity to Drechslera maydis T-toxin and the synthesis of a variant mitochondrial polypeptide in plants derived from maize tissue cultures with texas male-sterile cytoplasm. Theor Appl Genet. 1982;63(1):75-80.
[2] Levings CS., III. Cytoplasmic male sterility. Genet , eng. of Plants: Agr. Perspect. Proc. symp. New York; London, 1983:81-92.
[3] Kemble RJ, Mans RJ, Gabay-Laughnan S, Laughnan JR. Sequences homologous to episomal mitochondrial DNAs in the maize nuclear genome. Nature. 1983;304(5928):744–7.
[4] Hanson MR, Conde MF. Functioning and Variation of Cytoplasmic Genomes: Lessons from Cytoplasmic–Nuclear Interactions Affecting Male Fertility in Plants. Int Rev Cytol. 1985;213–67.
[5] Laughnan JR, Gabay-Laughnan S. Cytoplasmic male sterility in maize. Annu Rev Genet. 1983;17:27-48.
[6] Chase CD, Pring DR. Circular plasmid DNAs from mitochondria of Sorghum bicolor. Plant Mol Biol. 1985;5(5):303-11.
[7] Goblet JP, Boutry M, Duc G, Briquet M. Mitochondrial plasmid-like molecules in fertile and male sterileVicia faba L. Plant Mol Biol. 1983;2(6):305-9.
[8] Holford P, Newbury HJ, Groft H. Differences in the mitochondrial DNA of male-fertile, cms-S and cms-T onions. Eucarpia-Sec. Vegetables: Proc. 4th Allium Symp. (Coventry, 6-9 Sept., 1988). Wellssbourne, 1988:205-211.
[9] Perez C, Dujon B, Heizmann P, Bervillé A. Sequence of a mitochondrial plasmid of sunflower (Helianthus annuus) and its relationship to other mitochondrial plasmids. Plant Sci. 1988;58(1):59–69.
[10] Hanson MR, Young EG, Rothenberg M. Sequence and expression of a fused mitochondrial gene, associated with petunia cytoplasmic male sterility, compared with normal mitochondrial genes in fertile and sterile plants. Philos Trans R Soc Lond B Biol Sci. 1988;319(1193):199–208.
[11] Brendel V, Segel LA. On modes of recombination, replication, and segregation of the higher plant mitochondrial genome. J Theor Biol. 1987;125(2):163–76.
[12] Davis RW, Simon M, Davidson N. Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol. 1971;413–28.
[13] Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983;153(1):163-8.
[14] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.
[15] Ohta Y. High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc Natl Acad Sci U S A. 1986;83(3):715-9.
[16] Kemble RJ, Thompson RD. S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins. Nucleic Acids Res. 1982;10(24):8181-90.
[17] Meints RH, Hu W,Schuster A. et al. Electron microscopy study of the plasmid-like DNAs of maize mitochondria. Maydica. 1989; 34(3):197-205.
[18] Paillard M, Sederoff RR, Levings CS. Nucleotide sequence of the S-1 mitochondrial DNA from the S cytoplasm of maize. EMBO J. 1985;4(5):1125-8.
[19] Palzkill TG, Oliver SG, Newlon CS. DNA sequence analysis of ARS elements from chromosome III of Saccharomyces cerevisiae: identification of a new conserved sequence. Nucleic Acids Res. 1986;14(15):6247-64.
[20] Konstantinov YuM, Podsosonny VA, Lutsenko GN, Rivkin MI. DNA synthesis in intact maize mitochondria treated by pBR bacterial vector plasmids. Biopolym Cell. 1989; 5(4):98-102.