Biopolym. Cell. 2005; 21(3):270-274.
Molecular and Cell Biotechnologies
Development of model DNA-vaccine
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The development of DNA-vaccines is one of the most perspective directions in modern vaccionology. However, despite rapid progress in their application, a broad range of questions concerning the basic mechanisms of induction of immune response to DNA-vaccination remains unanswered. A model DNA-vaccine, containing the gene of Escherichia coli β-galactosidase as the gene of model antigen, has been developed. The data obtained suggest that the created recombinant plasmid pBG-TR/02 is able to induce the humoral immune response to E. coli β-galactosidase in mice depending on the place of administration and, therefore, it can be used as the model DNA-vaccine for future research.
Keywords: DNA-vaccine, model antigen, immunization, humoral immune response
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