Biopolym. Cell. 1997; 13(6):503-508.
Viruses and Cell
Studying of particular features of mixed infection of lymphocytes by HIV and some DNA-genomic lymphotropic viruses which may be associated with AIDS
1Dyachenko N. S., 2Rybalko S. L., 2Grytsak T. F., 2Maksimenok E. V., 2Djadiun S. T., 1Povnitsa O. Ju., 1Nesterova N. V., 1Beregovenko V. N., 3Pazkovsky Ju. V.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
  2. Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038
  3. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A model of mixed infection of lymphoblastic cells of T-phenotype was first designed using HIV and some DNA-genomic viruses which may be associated with AIDS (Epstein-Barr virus belonging to Herpesviridae and human adenovirus type 2). Considerable dec­reasing of HIV infection Hires as well as Ad hexon and EBV capsid proteins formation under triple (HIV+ Ad+ EBV) or double (HIV +Ad, HIV + EBV) infection of MT4 cells in different versions of non-simultaneous consecutive introduction of each virus were revealed, while HIV p24 expression levels were unchanged. Reverse transcriptase activity decreases upon triple infection of MT4/BIII cells chronically infected by HIV and producing it, while superinfection of cells by Ad increases level of enzyme. Drastic decreasing of interferone production was observed upon triple infection of lymphocytes from HIV-infected pacients and MT4 cells. Obtained data on mutual interference of HIV, Ad and EBV upon joint infection of cells are important for HIV-infection mechanism understanding, especially in conditions of latent lymphotropic viruses broad circulation among population of Ukraine in con­temporary ecological conditions.


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