Biopolym. Cell. 1993; 9(6):66-70.
Cell Biology
Resistance of in vitro germinating tomato pollen to kanamycin
1Chesnokov Yu. V., 1Shevkun O. B., 1Glushakova A. A.
  1. Institute of Genetics, Academy of Science of Moldova
    20, Padurii Str., Chisinau, Republic of Moldova, MD-2002


An influence of various kanamycin concentration on total tomato pollen grain germination percentage and tomato pollen tube length is described in the article. A correlation is established between tomato plant sporophyte and gametophyte with the trait of resistance to kanamycin. At the same time differences are distinquished by means of breeding at gametophyte level at the stage of mature pollen between homo-and heterozygous state of plants which are resistant to kanamycin. Possible effects of kanamycin action on physiological and genetical activity of germinating pollen are discussed in various (resistant and susceptible to kanamycin) tomato genotypes.


[1] Willing RP, Mascarenhas JP. Analysis of the complexity and diversity of mRNAs from pollen and shoots of tradescantia. Plant Physiol. 1984;75(3):865-8.
[2] Mascarenhas JP, Mermelstein J. Messenger RNas : their utilization and degradation during pollen germination and tube growth. Acta Societatis Botanicorum Poloniae. 1981;50(1-2):13–20.
[3] Mascarenhas NT, Bashe D, Eisenberg A, Willing RP, Xiao CM, Mascarenhas JP. Messenger RNAs in corn pollen and protein synthesis during germination and pollen tube growth. Theor Appl Genet. 1984;68(4):323-6.
[4] Tup? J. Alterations in polyadenylated RNA during pollen maturation and germination. Biol Plant. 1982;24(5):331–40.
[5] Tanksley SD, Zamir D, Rick CM. Evidence for Extensive Overlap of Sporophytic and Gametophytic Gene Expression in Lycopersicon esculentum. Science. 1981;213(4506):453-5.
[6] Sari Gorla M, Frova C, Binelli G, Ottaviano E. The extent of gametophytic-sporophytic gene expression in maize. Theor Appl Genet. 1986;72(1):42-7.
[7] Rajora OP, Szuffa L. Sporophytic and gametophytic gene expression in Populus deltoid cs Marsh., P. nigra L. and P. maximowiezii Henry. Can J Genet Cytol. 1986; 28:476-82.
[8] Chalyk ST, Lyakh VA, Pernulyak LP. New approaches in maize breeding. Kishinev: Shtiintsa, 1992; 56-117.
[9] Bino RJ, Hille J, Franken J. Kanamycin resistance during in vitro development of pollen from transgenic tomato plants. Plant Cell Rep. 1987;6(5):333-6.
[10] Chesnokov YV Ability genetic transformation of plants using the process of pollination, fertilization: Author. Dis .... kand. biol. Sciences. Minsk, 1990. 16 p.
[11] Chesnokov YuV, Sedov GI, Vikonskaya NA. A simple method for genetic transformation of dicotyledonous plants. Izv Akad Nauk MoldSSR. 1989; 6: 61-2.
[12] Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugawara Y. Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet. 1986;204(2):204–7.
[13] Zaytsev GN. Methodology biometric calculations. Nauka, 1973. 256 p