Biopolym. Cell. 1993; 9(6):63-66.
The purification of the main tissue-specific antigens from the normal and effected by dilatative cardiomyopathia human heart
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- M. D. Strazhesko Institute of Cardiology, MAS of Ukraine
5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
The methods for the purification of the main tissue-specific antigens-(actin, myosin and tropomyosin) from the normal and affected by dilatative cardiomyopathia human heart are described. Some their physico-chemical properties were investigated. The dicrease of the contractive proteins content in the DCMP myocard in comparisons with the normal was shown.
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 von Lothar Jager. Klinische Immunologie und Allergologie. Jena, 1988
 Rose NR, Neumann DA, Herskowitz A. Autoimmune myocarditis: concepts and questions. Immunol Today. 1991;12(8):253-5.
 Cederholm B, Wieslander J, Bygren P, Heineg?rd D. Patients with IgA nephropathy have circulating anti-basement membrane antibodies reacting with structures common to collagen I, II, and IV. Proc Natl Acad Sci U S A. 1986;83(16):6151-5.
 Grant MD, Weaver MS, Tsoukas C, Hoffmann GW. Distribution of antibodies against denatured collagen in AIDS risk groups and homosexual AIDS patients suggests a link between autoimmunity and the immunopathogenesis of AIDS. J Immunol. 1990;144(4):1241-50.
 Dan-Goor M, Muhlrad A. Antibody directed against the 142-148 sequence of the myosin heavy chain interferes with myosin-actin interaction. Biochemistry. 1991;30(2):400-5.
 Spudich JA, Watt S. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J Biol Chem. 1971;246(15):4866-71.
 Epstein HF, Fischman DA. Molecular analysis of protein assembly in muscle development. Science. 1991;251(4997):1039-44.
 Ivanovskiy YUV, Nagornev VA, Zubzhitskiy YUN. Preparation of antisera against antigens aortic immunomorphological experimental study of atherosclerosis. Bull Exp Biol Med. 1975; 3:72-7.
 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
 Moore LA, Arrizubieta MJ, Tidyman WE, Herman LA, Bandman E. Analysis of the chicken fast myosin heavy chain family. Localization of isoform-specific antibody epitopes and regions of divergence. J Mol Biol. 1992;225(4):1143-51.