Biopolym. Cell. 1993; 9(6):59-62.
http://dx.doi.org/10.7124/bc.000382
Structure and Function of Biopolymers
Determination of temperatures of structural-phase reconstructions in model and biomembranes from spectral characteristics of merocyaninc 540
1Boldeskul A. E., 1Velichko A. N.
  1. Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
    14, Eugene Pottier Str., Kyiv, Ukraine, 03057

Abstract

Absorption and fluorescence spectra of cyanine dye merocyanine 540 (M540) in organic solvents, liposomes from different classes of lipids, in rat liver microsomes and erythrocytes were studied. It was shown that together with the known way of determination of temperatures of phase-transitions Tn from absorption spectra of M540, determination of Tn from quantum yield of monomers was possible; at the same time the method proposed was more simple. Comparison of both methods was carried out.
Full text: (PDF, in Russian)

References

[1] Ivkov VG, Berestovskiy GN. The lipid bilayer of biological membranes. M. Nauka, 1982. 224 p.
[2] Ivkov VG, Berestovskiy GN. Dynamic structure of the lipid bilayer. M.: Nauka, 1981. 296 p.
[3] Dobretsov GE. Fluorescent probes in the study of cell membranes and lipoproteins. Moscow: Nauka, 1989. 277 p.
[4] Waggoner AS. Dye indicators of membrane potential. Annu Rev Biophys Bioeng. 1979;8:47-68.
[5] GubskiД­ IuI, Boldeskul AE, Primak RG, Zadorina OV. The effect of alpha-tocopherol and ionol on the physical structure of the membranes of rat liver microsomes under conditions of antioxidant insufficiency. Ukr Biokhim Zh. 1989;61(4):94-9.
[6] Gordon A, Ford R. Satellite chemist. M : Mir, 1976. 544 p.
[7] Lelkes PI, Miller IR. Perturbations of membrane structure by optical probes: I. Location and structural sensitivity of merocyanine 540 bound to phospholipid membranes. J Membr Biol. 1980;52(1):1-15.
[8] Shikurova L, Haban I, Chorvat DI. Dimers of merocyanine 540 in aqueous solutions. Stud Biophys. 1988; 125:107-11.
[9] Ishchenko AA, Mushkalo IL, Derevyanko NA. et al.Association bis cyanine dyes with tightly coupled chromophores in solvents of different polarity. J Inf Rec Mater. 1989; 17: 39-51.
[10] Glushko V, Thaler MS, Karp CD. Pyrene fluorescence fine structure as a polarity probe of hydrophobic regions: behavior in model solvents. Arch Biochem Biophys. 1981;210(1):33-42.
[11] Verkman AS, Frosch MP. Temperature-jump studies of merocyanine 540 relaxation kinetics in lipid bilayer membranes. Biochemistry. 1985;24(25):7117-22.
[12] Biological Membranes: A Practical Approach (The Practical Approach Series). Ed J. B. C. Findlay. Oxford University Press; 1987; 320 p.
[13] Aramendia PF, Krieg M, Nitsch C, Bittersmann E, Braslavsky SE. The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes. Photochem Photobiol. 1988;48(2):187-94.
[14] Itogi nauki i tekhniki. Biophysics of membranes. Ed. R. I. Zhdanova, AM Komarova. M. : VINITI, 1989. vol. 6. 210 p.