Biopolym. Cell. 1993; 9(6):13-21.
Biochemical characteristics of the rat liver transcriptionally active and repressed chromatin
1Levitsky E. L., 1, 2Gubsky Yu. I., 3Chabanny V. N., 2Volkov G. L., 3Novikova S. N.
  1. Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
    14, Eugene Pottier Str., Kyiv, Ukraine, 03057
  2. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601
  3. Gerontology Institute of the Academy of Medical Sciences of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114


The intact rat liver trancriptionally active and repressed chromatin fractions have been characterized. The transcriptionally activity differences between the fractions may be conditioned by distinctions in the chromatin protein component, that is increased RNA-polymerases concentration in active chromatin. Differences between in vivo and in vitro DNA synthesis, thermodenaturation process and susceptibility to endo- and exogenous DNA-ases are insignificant and so it may be thought the DNA structure similarity in active and repressed chromatin. Moreover it may be thought that differences between phospholipid and fatty acids fractions compositions may be a cause of the chromatin realization of its genetic activity.


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