Biopolym. Cell. 1993; 9(5):89-100.
http://dx.doi.org/10.7124/bc.000378
Gene-Engineering Biotechnology
The interphase chromatin special state zones
1Shpilevaya S. P., 1Kostetsky I. E., 1Bilitch K. M., 1Lihatcheva L. I., 1Stolar T. V., 1Irodov D. M., 1Kostetskaya E. V., 1Spivak I. M., 1Zhestyanikov V. D., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The possibility is demonstrated of RecA protein of E. coli introduction into eukaryotic cell with preservation of its biologicall activity. The character of this protein affinity to chromatin of different degree of condensation is shown on cytological level (undirect immunofluorescent method combined with cytophotometry) for different stages of cellular cycle (protein is not tested in meta- and anaphase chromosomes). The presence of bacterial RecA protein in the nuclei and cytoplasm of cells both in vitro and in vivo is confirmed with the help of immunoelectron microscopy. Studies were carried out on cultures of HeLa and Ltk– cells and also in vivo on hepatocytes after direct injection on RecA protein and plasmid pKCR2 enclosed in liposomes into the liver of adult mice line BALB/c. Proceeding from the experimental data obtained and also considering the fact that RecA protein mainly connects with single-stranded DNA, the assumption is done about existence of special state chromatin zones (SSCZ). For these zones the active affinity to RecA protein serum is character with intensive fluorescence, thus they may correspond to some actively expressing genes, gathered in clusters.
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