Biopolym. Cell. 1993; 9(5):73-77.
Cell Biology
Genetic transformation of some Solanum and Lycopersicon wild species by leaf-discs method
1Rudas V. A., 1Zavgorodnya A. V., 1Cherep N. N., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


Using Agrobacterium tumefaciens-mediated transformation kanamycin-resistant plants of Lycopersicon peruvianutn var. humifusum, L. peruvianum Mill., L. pennellii, Solatium aculeatissimum, S. sisymbrifolium, S. gilo, S. mammosum were obtained. By this procedure plants of L. peruvianum var. dentatum, S. rickil, S. lycopersicoides, S. nigrum resistant to kanamycin, hygromycin as well as resistance to both kanamycin and hygromycin were obtained too. Assay of neornycin phosphotransferase (NPTII)-activity confirmed this chimaeric gene expression in the transformed plants.


[1] Bellini C, Chupeau M-C, Guerche P, Vastra G, Chupeau Y. Transformation of Lycopersicon peruvianum and Lycopersicon esculentum mesophyll protoplasts by electroporation. Plant Sci. 1989;65(1):63–75.
[2] Dix PJ, Kinley CP, Cabe PF. Antibiotic Resistant Mutants of Solanum Nigrum. Progress in Plant Cellular and Molecular Biology. 1990;169–74.
[3] Rudas VA. Getting chlorophyll-deficient and transgenic plants in the family Solcinaceae. Tez. of reports. conf. young scientists "Actual problems of Plant Physiology and Genetics (26-28 May 1992, Kiev). Kyiv, 1992: 139.
[4] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[5] Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50(1):151-8.
[6] Sidorov VA, Piven NM, Gleba YuYu, Sytnik KM. Solanaceae somatic hybridization. Kiev: Naukova Dumka. 1985; 132 p.
[7] Schreier PH, Seftor EA, Schell J, Bohnert HJ. The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J. 1985;4(1):25-32.