Biopolym. Cell. 1993; 9(4):39-44.
http://dx.doi.org/10.7124/bc.000364
Structure and Function of Biopolymers
Analysis of antigenic properties of B. pertussis filamentous hemagglutinin RGD-containing site responsible for the reaction with macrophages
1Pkhakadze H. G., 1Kavun E. M., 1Chechet V. A., 1Marinets A. V., 2Radavsky Yu. L., 1Komissarenko S. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

The antigen determinant distinguished by the antibodies was found in the region 1094–1102 of filamentous hemagglutinin from B. pertussis. The native FHA, nonapeptide TVQRGDPHQ, its conjugates with albumins from bovine and human sera were used in the experiments. The first conjugate was obtained with the glutaral-dehid, the another one with the use of SPDP. The immurioaffinity chromatography and ELISA were used for the determination of the epitope-specific antibodies. Localization of the antigen determinant in this site of FHA makes it possible to apply the peptide as a component of the synthetic vaccine against whooping-cough.
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