Biopolym. Cell. 1993; 9(3):27-34.
Structure and Function of Biopolymers
Influence of vitamin E on structural and functional liver chromatin organization during tetrachloromethane damage
1Gubsky Yu. I., 1Levitsky E. L., 1Primak R. G., 1Goriushko A. G., 1Lenchevskaya L. K., 1Matushrin V. A., 1Vistunova I. E., 1Sachenko L. G.
  1. Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
    14, Eugene Pottier Str., Kyiv, Ukraine, 03057

Abstract

Partial corrective influence of vitamin E introduction on structural and functional liver nuclear chromatin organization which was damaged during tetra-chloromethane intoxication was shown. This one is depended on α-tocopherol antioxidant effects that displais as normalization of spontaneous and inducted lipid peroxidation reactions in active and repressed chromatin fractions. Introduction of vitamin E leads also to partial normalization of endogenous DNA polymerase β and RNA polymerases as well as stimulation of replicative DNA polymerase a activity in active chromatin fraction. In vitro interaction process of α-tocopherol with chromatin fractions has written. Tetrachloromethane intoxication has little influence on repressed chromatin ability to form complexes with vitamine E, but increases its binding to active chromatin fraction. Differences between in vitro chemical interaction of active and repressed chromatin fractions with α-tocopherol were revealed. These ones indicate to various localization of vitamin E in chromatin. Interaction of vitamin E with chromatin leads to partial repair of its structure damaged during intoxication. This effect of vitamin E together with its antioxidant action is one of the possible mechanism of α-tocopherol genoprotective influence.

References

[1] Khalmuradov AG, Tonkiy VI, Chagovets RV. Transport fat-soluble vitamins. Kiev: Naukova Dumka, 1980. 216 p.
[2] Gubskiĭ IuI, Levitskiĭ EL, Chabannyĭ VN, Gol'dshteĭn NB, Kapralov AA, Petrova GV, Donchenko GV, Volkov GL, Litoshenko AIa. Changes in protein, lipid composition, DNA- and RNA- polymerase activity of the chromatin fraction and the nuclear matrix of the rat liver in hypovitaminosis E. Ukr Biokhim Zh. 1990;62(6):22-30.
[3] Gubskiy JuI, Levitsky EL, Primak RG, Velichko AN. Change in the structural condition of the fractionated liver chromatin under lipid peroxidation activation. Biopolym Cell. 1991; 7(3):89-94.
[4] Donchenko GV, Petrova GV, Kapralov AA, Gubsky YuI, Levitsky EL. Possible role of the a-tocopherol in functioning of chromatin in nuclear matrix in the rat liver. Doklady Akad Nauk Ukr SSR. Ser B. 1990; (3):60-2.
[5] Gubskiĭ IuI, Levitskiĭ EL, Gol'dshteĭn NB, Mozzhukhina TG, Litoshenko AIa, Novikova SN. Functional activity of fractionated chromatin from rat liver upon a single administration of carbon tetrachloride. Vopr Med Khim. 1989;35(4):119-24.
[6] Gubskiĭ IuI, Levitskiĭ EL, Primak RG, Golubov MI, Novikova SN. Conformational characteristics and packing of endogenous lipid fractions of transcriptionally active and repressed chromatin. Ukr Biokhim Zh. 1991;63(2):83-9.
[7] Sverdlova OV. Electronic spectra in organic chemistry. L: Khimiya, 1985. 248 p.
[8] Koroliuk MA, Ivanova LI, Maĭorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9.
[9] Ashmarin IP, Vasil'yev II, Ambrosov VA. Rapid methods of statistical processing and planning experiments. L. : Izd-vo Leningr. gos univ-ta. 1975; 78 p.
[10] Gubskiĭ IuI, Levitskiĭ EL, Gol'dshteĭn NB, Litoshenko AIa. Lipid peroxidation and the endogenous DNA polymerase activity of fractions of isolated liver chromatin in rats. Biull Eksp Biol Med. 1989;107(3):296-8.
[11] Kaznacheev IuS, Kulagina TP, Markevich LN, Kolomiĭtseva IK, Kuzin AM. Metabolism of chromatin lipids in rat liver and thymus. Mol Biol (Mosk). 1984;18(3):607-12.
[12] Demchenko AP, Zima VL. UV spectrophotometry and the structure of proteins. Kyiv, Naukova Dumka, 1981; 208 p.
[13] Sivolob AV, Khrapunov SN. Fluorescence spectroscopy in the investigations of protein-nucleic acids interactions in chromatin. Biopolym Cell. 1992; 8(1):89-100.
[14] Gubskiĭ IuI, Boldeskul AE, Primak RG, Zadorina OV. The effect of alpha-tocopherol and ionol on the physical structure of the membranes of rat liver microsomes under conditions of antioxidant insufficiency. Ukr Biokhim Zh. 1989;61(4):94-9.