Biopolym. Cell. 1993; 9(1):26-31.
http://dx.doi.org/10.7124/bc.000349
Cell Biology
Transformation of plant Nicotiana tabacum with a gene coding for DNA Topoisomerase II type from Drosophila melanogaster
1Storozhenko S. V., 1Shisha H. N., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

A plasmid pBin19/t2103 based on a binary vector pBin19 has been constructed. The plasmid carryes the structural gene coding for DNA topoisomerase II type from D. melanogaster under control of 35S promoter from cauliflower mosaic virus. The construction was introduced into tobacco plants N. tabacum SR-1 using A. tumefaciens-mediated transformation. An integration of the gene in plant genome and its expression on transcriptional level have been shown. A sequence from tobacco genome was found to hybridized with the fragment of the topoisomerase II structural gene. It seems to be plant DNA topoisomerase II type gene.
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