Biopolym. Cell. 1992; 8(6):55-57.
Gene-Engineering Biotechnology
Cloning in the laclq of pTTQ plasmids: «reverse» colour reaction
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
The method for cloning Mlul-, BclI-, ApaI-, BssHI- and EcoRV-DNA fragments in the laclq gene of pTTQ plasmids is described. Recombinant clones are selected as coloured in blue colour colonies on plates containing ampicillin and X-gal.
Full text: (PDF, in Russian)
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