Biopolym. Cell. 1992; 8(6):44-49.
Cell Biology
Transformation of some potato cultivars and analysis of transgenic plants
1Kravetz O. A., 1Pogrebnyak N. Y., 1Shisha Ye. N., 1Lopato S. V., 1Gleba Yu. Yu.
- Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
Abstract
Kanamycin resistant plants of Solanum tuberosum L. (cv. Lugovskoy, Desiree) and Nicotiana tabacum SR-1 were obtained following the cocultivation of stem segments and leaf discs with Agrobacterium tumefaciens. A disarmed binary vector system containing the neomycin phosphotransferase (NPT II) gene and gene encoding mutant acetolactate synthase (ALS) from Arabidopsis thaliana was used. Transformation was confirmed by the ability of stem segments to initiate callus on medium containing 100 mg/1 kanamycin and Southern blot analysis.
Full text: (PDF, in Russian)
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