Biopolym. Cell. 1992; 8(5):48-54.
Gene-Engineering Biotechnology
pZE8 natural hsd plasmid replicon based cloning vectors for Escherichia coli and endorhizosphere nitrogen fixer Klebsiella oxytoca VN13
1Alexeyev M. F., 1Kovtunovich G. L., 1Kravetz A. N., 2Solonin A. S.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Skryabin Institute of Biochemistry and Physiology of Microorganisms Russian Academy of Science
5, Prospect Nauki, Puschino, Moscow reg., , Russian Federation,142290
Abstract
Natural Citobacter freundii hsd plasmid based vector plasmids for Klebstella oxytoca VN13 and Escherichia coli have been constructed. pKAS18 and pKAS19B vectors are consist of pZE8 ori, Tn903 Kmr gene and polylinkers of pUC18 or pUC19 respectively. pMG1k and pMG21k plasmids allow cloning into the unique PstI site of the EcoRV restrictase gene.
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