Biopolym. Cell. 1992; 8(5):16-20.
http://dx.doi.org/10.7124/bc.000334
Structure and Function of Biopolymers
Oligonucleopeptides. II. Synthesis of oligonucleotidyl-(P→N)-peptides using oxidation-reduction reagent-triphenylphosphine, 2,2'-dipyridyldisulfide
1Yarmoluk S. M., 1Korol L. S., 1Alekseeva I. V., 1Shalamay A. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

We studied the formation of P→N bond in the oligonucleopeptides of phosphoramide type with use of oxidation-reduction reagent – triphenylphosphine, 2,2'- dipyridyldisulfide in the presence of various nucleophilic agents. Reaction depended on pKa of iaminogroup of peptides. Highest yields were obtained using peptides with pKa about 9. The method allows to prepare oligonucleopeptides bouth at 31'- and 5'-terminal phosphate groups.
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