Identification of phosphate residues involved in the formation of tertiary structure of tRNA1Ser from bovine liver

Kalachnyuk, L. G.; Tukalo, M. A.; Matsuka, G. Kh.

doi:10.7124/bc.000333

Biopolym. Cell. 1992; 8(5):12-15.

http://dx.doi.org/10.7124/bc.000333

Structure and Function of Biopolymers

Identification of phosphate residues involved in the formation of tertiary structure of tRNA₁^Ser from bovine liver

1Kalachnyuk L. G., 1Tukalo M. A., 1Matsuka G. Kh.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The phosphates of the tRNA₁^Ser from bovine liver (tRNA which has a long variable loop) participating in the formation of three dimensional structure were studied by alkylation modification was observed for the following phosphates: 8, 9, 11 (at the bend site between the acceptor and D-stem), 21- in the D-loop, 47 G, 47 I and 49 in the variable stem and at the joint of variable and T-stem. 56, 58, 59 in the T-loop. The variable arm of tRNA₁^Ser from bovine liver is exposed in solution.

Full text: (PDF, in Russian)

References

[1] Kisselev LL, Favorova OO, Lavrik OI. Biosynthesis of proteins from amino acids to aminoacyl-tRNA. Moscow, Nauka, 1984; 408 p.

[2] Tukalo MA. Primary and spatial structure of tRNA. Mol Biol (Mosk). 1984;18(5):1233-48.

[3] Vlassov VV, Giegé R, Ebel JP. Tertiary structure of tRNAs in solution monitored by phosphodiester modification with ethylnitrosourea. Eur J Biochem. 1981;119(1):51-9.

[4] Vlassov VV, Giege R, Ebel JP. The tertiary structure of yeast tRNAPhe in solution studied by phosphodiester bond modification with ethylnitrosourea. FEBS Lett. 1980;120(1):12-6.

[5] Kalachniuk LG, Tukalo MA, Matsuka GKh. Primary structure of tRNA1ser from bovine liver. Ukr Biokhim Zh. 1989;61(1):72-5.

[6] Kalachnyuk LG, Kozak LA, Tukalo MA, Matsuka GKh. Isolation and characteristic of the individual isoacceptor tRNA1Ser preparation from the bovine liver. Biopolym Cell. 1987; 3(5):274-6.

[7] Petrushenko ZM, Tukalo MA, Matsuka GKh. Determination of phosphate residues participating in the formation of the spatial structure of tRNA- Leu IAG from cow mammary glands. Bioorg Khim. 1986;12(11):1492-7.

[8] Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977;4(8):2527-38.

[9] Dock-Bregeon AC, Westhof E, Giegé R, Moras D. Solution structure of a tRNA with a large variable region: yeast tRNASer. J Mol Biol. 1989;206(4):707-22.