Biopolym. Cell. 1992; 8(4):56-58.
http://dx.doi.org/10.7124/bc.000331
Gene-Engineering Biotechnology
Replication of oncogene c-myc in Ehrlich ascitic cells
1Danko I. M., 2Prima V. I.
  1. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Sedimentational fractionation of asynchronic Ehrlich ascitic cells po¬pulation was carried out according to cell cycle phases. Separation of tumor cells suspension was carried out under 1 g in 0–0,3 M sucrose concentration gradient. Cells, pituated in S phase, were selected to 3 fractions in early, midle and late S phase respectively. It was studied temporal order replications of synchronized Ehrlich ascitic cells. It was shown that the replication of oncogene c-myc beginning in early S phase.
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