Biopolym. Cell. 1992; 8(4):30-33.
http://dx.doi.org/10.7124/bc.00032B
Structure and Function of Biopolymers
Isolation of active ribulose-1,5-bisphosphate carboxylase from cotton leaves of Gossypium hirsutum and G. barbadense L. species
1Karpilova I. Ph., 2Sokolova I. M., 1Gaziyants S. M.
  1. Institute of Soil Science and Photosynthesis RAS
    2, Istitutskaya Str., Pushchino, Moscow Region, Russian Federation, 142290
  2. Institute of Experimental Biology of Plants, Academy of Sciences of the Republic of Uzbekistan
    Uzbekistan

Abstract

A rapid method of isolation of ribulose-1,5-bisphosphate carboxylase from cotton leaves is offered. Definite complex of additional substances completely bio-cades the polyphenoles and allows to obtain high level of common and specific activities of Rubisco isolated from adult cotton leaves with high content of phenolic substances. Method is useful for the comparative analysis of the examples seria and may be used for the genetic and evolutional investigations.
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