Biopolym. Cell. 1992; 8(3):53-56.
http://dx.doi.org/10.7124/bc.000328
Short Communications
The effect of neomycin on poly(U) and poly(dT) translation in cell-free systems from Saccharomyces cerevisiae and Neurospora crassa
1Shul'ga N. I., 1Potapov A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The effect of different concentration of neomycin on poly(U) and poly(dT) translation has been studied in cell-free systems from S. cerevisiae and M. crassa. The data show that this translation is carried out by cytoplasmic ribosomes. Mg2+ iones stimulate poly(U) and poly(dT) translation in these systems and neomycin unlike the prokaryotic cell-free systems inhibits translation of both templates. It was proposed that ribosome decoding center was changing during evolution to higher stereospecificity to the codon-anticodon sugar-phosphate backbone structure.
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References

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