Biopolym. Cell. 1992; 8(3):46-50.
http://dx.doi.org/10.7124/bc.000326
Gene-Engineering Biotechnology
Method for rapid transformation of enterobacteria and some factors influencing its efficiency
1Alexeyev M. F., 1Gunkovskaya N. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Standard and simplified modifications of freese-thaw method for enterobacteria transformation are described. First enables one to transform E. coli JM109 and K. oxytoca VN13 within 1 hour with an efficiency of about 2.5·105 transformants per microgram of plasmid pUC4k DNA. Simplified modification gives an efficiency of 1·104 transformants per microgram of pUC4k DNA within 5 min. Both modifications were successfully applied for the transformation of Enterobacter cloaceae JMV8351.
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