Biopolym. Cell. 1992; 8(3):33-36.
http://dx.doi.org/10.7124/bc.000322
Cell Biology
Optimization of the system for genetic-embryological studies of inbred mice strains. 2. Comparative characterization of season female fertility of BALB/cLac, C57BL/6j and ICR mice
1Morozova L. M., 1Stolina M. R., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

We present data on optimization of the biological system for in vitro fertilization without preliminary hormonal stimulation of inbred ova donors. An incubation, medium for in vitro fertilization was chosen, which is optimal for it least two different in many features inbred mice strains C57BL/6j and BALB/cLac. We demonstrated the male genotype is dominant during in vitro fertilization, while the female genotype appears to be dominant in the course of zygote development in culture until late preimplantation stages. Application of C57BL spermatozoa rises the effectivity of in vitro fertilization from 50 to 76 %. Maternal C57BL genotype ensures successful development of about 90 % of homo- and heterozygotes till blastocyst stage
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References

[1] Whittingham DG. Fertilization of mouse eggs in vitro. Nature. 1968;220(5167):592-3.
[2] Pavlok A. Fertilization of mouse ova in vitro. I. Effect of some factors on fertilization. J Reprod Fertil. 1968;16(3):401-8.
[3] Hoppe PC, Pitts S. Fertilization in vitro and development of mouse ova. Biol Reprod. 1973;8(4):420-6.
[4] Fraser LR, Drury LM. The relationship between sperm concentration and fertilization in vitro of mouse eggs. Biol Reprod. 1975;13(5):513-8.
[5] Fraser LR. Motility patterns in mouse spermatozoa before and after capacitation. J Exp Zool. 1977;202(3):439-44.
[6] Storey BT, Lee MA, Muller C, Ward CR, Wirtshafter DG. Binding of mouse spermatozoa to the zonae pellucidae of mouse eggs in cumulus: evidence that the acrosomes remain substantially intact. Biol Reprod. 1984;31(5):1119-28.
[7] Talansky BE, Gordon JW. Cleavage characteristics of mouse embryos inseminated and cultured after zona pellucida drilling. Gamete Res. 1988;21(3):277-87.
[8] Blandova ZhK, Dushkin VA, Malashenko AM, Shmidt YeF. Lines of laboratory animals for biomedical research. Moscow, Nauka, 1983; 192 p.
[9] Hogan B, Constantini F, Lacy E. Manipulating the mouse embryo. A laboratory manual. New York : Cold Spring Harbor Lab., 1986; 350 p.
[10] Fraser LR. Potassium ions modulate expression of mouse sperm fertilizing ability, acrosome reaction and hyperactivated motility in vitro. J Reprod Fertil. 1983;69(2):539-53.
[11] Bander SA, Watson SC, Shire JG. Paternal inheritance of egg traits in mice: a case of genomic imprinting. Genet Res. 1989;54(3):213-9.
[12] Seredenin SB, Rybina IV, Khlopushina TG, Zherdev VP. The determination of the oxidation phenotype in inbred C57Bl/6 and BALB/c mice. Biull Eksp Biol Med. 1990;110(11):491-3.
[13] Evsykov SV, Morozova LM, Solomko AP. Role of the nucleocytoplasmic ratio in the regulation of the mammal development. Development of zygotes with a decreased cytoplasm volume. Biopolym Cell. 1989; 5(5):87-93.