Biopolym. Cell. 1992; 8(1):89-100.
http://dx.doi.org/10.7124/bc.000315
Structure and Function of Biopolymers
Fluorescence spectroscopy in the investigations of protein-nucleic acids interactions in chromatin
1Sivolob A. V., 1Khrapunov S. N.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033

Abstract

The brief review dealt with the investigations of chromatin molecular organization by the fluorescent methods. The results of chromatin and nucleosome studies, which have been obtained by using of proteins and DNA labelling by fluorescent dyes, as well as the data of intrinsic protein fluorescence obtained mainly by the authors, are described. The advantages of the different fluorescent approaches, which allow to obtain a unique information about protein-nucleic acids complexes structure and dynamics, are demonstrated and discussed.
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