Biopolym. Cell. 1992; 8(1):56-62.
http://dx.doi.org/10.7124/bc.000310
Structure and Function of Biopolymers
Spectrophotometry analysis of behaviour of DNA solutions in the water and at low ionic strengths
1Kruglova E. B.
  1. A. Usikov Institute of Radio Physics and Electronics, NAS of Ukraine
    12, Proskura Str., Kharkov, Ukraine, 61085

Abstract

Dependences of the observed hyperchromism on DNA concentrations for DNA with different bases contents in the water were studied by UV-spectroscopy. It has been shown that DNA protonation begins with AT-pairs in the water and at Iow ionic strengths. The dependences of protonation constants values of AT-pair K1 on counterion concentration were obtained. It was carried out the comparison K1 with protonation constant of GC-pair K2 at different ionic strengths. Experimental data earlier obtained on the basis of proposed mechanism of protonation are discussed.
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