Biopolym. Cell. 1991; 7(6):91-95.
Structure and Function of Biopolymers
Uridine-diphosphate-N-Acetyl-muramyl-L-alanyl-d-glutamate: meso-2, 6-diaminopimelate ligase activity in extracts of Basillus subtilis, Streptococcus bovis and Enterococcus faecium
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate: meso-2, 6-diaminopimelate ligase activity was found in extracts of B. subtilis, S. bovis, E. faecium. UDP-N-acetylmuramyl-L-alanyl-D-glutamate: meso-2, 6-diaminopimelate ligase from B. subtilis was purified by gel-filtration and ion-exchange chromatography. The molecular weight of enzyme as determined by gel-filtration is 120000.
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