Analysis of asymmetric somatic hybrid genome organization in Nicotiana

Korostash, M. A.; Butsko, E. V.; Kovtun, E. V.; Parokonnyy, A. S.

doi:10.7124/bc.0002F3

Biopolym. Cell. 1991; 7(5):55-62.

http://dx.doi.org/10.7124/bc.0002F3

Cell Biology

Analysis of asymmetric somatic hybrid genome organization in Nicotiana

1Korostash M. A., 1Butsko E. V., 1Kovtun E. V., 1Parokonnyy A. S.

Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

Inter- and intraspecific «gamma»-hybrids in Nicctiana were obtained with dose usage 10–1000 Gy. Cytological and molecularbiological analysises of 60 hybrid lines showed, that polyploid «gamma»-hybrids, containing one irradiated donor genome per two, three intact recipient ones, have selective advantages. 75–96 % donor chromosomes didn't preserve in fusion products after treatment with 1000 Gy.

Full text: (PDF, in Russian)

References

[1] Aviv D, Chen R, Galun E. Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids? Plant Cell Rep. 1986;5(3):227-30.

[2] Dudits D, Fejer O, Hadlaczky G, Koncz C, Lazar GB, Horvath G. Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet. 1980;179(2):283–8.

[3] Gupta PP, Schieder O, Gupta M. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet. 1984;197(1):30–5.

[4] O'Connell MA, Hanson MR. Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solatium rickii. Theor Appl Genet. 1986;72(1):59-65.

[5] Glimelius K, Bonnett HT. Nicotiana cybrids with Petunia chloroplasts. Theor Appl Genet. 1986;72(6):794-8.

[6] Sidorov VA, Zubko MK, Kuchko AA, Komarnitsky IK, Gleba YY. Somatic hybridization in potato: use of γ-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction. Theor Appl Genet. 1987;74(3):364–8.

[7] Somers DA, Narayanan KR, Kleinhofs A, Cooper-Bland S, Cocking EC. Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion. Mol Gen Genet. 1986;204(2):296–301.

[8] Imamura J, Saul MW, Potrykus I. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor Appl Genet. 1987;74(4):445-50.

[9] Dudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey J, Cella R. Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants. Proc Natl Acad Sci U S A. 1987;84(23):8434-8.

[10] Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by "gamma-fusion". Theor Appl Genet. 1988;76(5):760-6.

[11] Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet. 1987;74(6):718-26.

[12] Dirks R, Negrutiu I, Heinderyckx M, Jacobs M. Genetic analysis of revertants for the nitrate reductase function of Nicotiana plumbaginifolia. Mol Gen Genet. 1986;202(2):309–11.

[13] Negrutiu I, Dirks R, Jacobs M. Regeneration of fully nitrate reductase deficient mutants from protoplast culture of Nicotiana plumbaginifolia (Viviani). Theor Appl Genet. 1983;66-66(3-4):341-7.

[14] Negrutiu I, De Brouwer D, Watts JW, Sidorov VI, Dirks R, Jacobs M. Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia, Viviani. Theor Appl Genet. 1986;72(2):279-86.

[15] Marton L, Dung TM, Mendel RR, Maliga P. Nitrate reductase deficient cell lines from haploid protoplast cultures ofNicotiana plumbaginifolia. Mol Gen Genet. 1982;186(3):301–4.

[16] Koukalová B, Reich J, Matyášek R, Kuhrová V, Bezděk M. A BamHI family of highly repeated DNA sequences of Nicotiana tabacum. Theor Appl Genet. 1989;78(1):77-80.

[17] Dellaporta SL, Wood J, Hicks JB. A plant DNA minipreparation: Version II. Plant Mol Biol Rep. 1983;1(4):19–21.

[18] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.

[19] Famelaer I, Yu Gleba Y, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, et al. Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by "gamma fusion". Plant Sci. 1989;61(1):105–17.

[20] Famelaer I, Negrutiu I, Mouras A, Vaucheret H, Jacobs M. Asymmetric hybridization in Nicotiana by "gamma fusion" and progeny analysis of self-fertile hybrids. Theor Appl Genet. 1990;79(4):513-20.