Biopolym. Cell. 1991; 7(5):37-41.
http://dx.doi.org/10.7124/bc.0002F0
Structure and Function of Biopolymers
The effect of modification of terminal groups of oligonucleotides on their stability in mycoplasma culture
1Vlassov V. V., 1Zarytova V. F., 1Ivanova E. M., 1Krendelev Yu. D., 2Ovander M. N., 1Ryte A. S.
  1. Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
    8, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The effect of modification of terminal groups of deoxyribooligonucleotides on their stability in culture medium and mycoplasma cells A. laldlawii PQ-8 and M. capricolum California kid has been investigated. The oligonucleotides and their derivatives were stable in culture medium with 10 % horse serum during 24 hours. In the medium with mycoplasms, orthophosphates were rapidly removed from the 5'-ends and the oligonucleotides degraded to mononucleotides. In the cells, the scission of 5'-phosphomono-ester bonds was accompanied by reutilization of the phosphate. Attachment of the cholesterol or phenazinium residues to oligonucleotides 3'- or 5'-ends protected them from nucleases. The modification of oligonucleotides 5'-ends by 5'-phosphoramides formation, protect the oligonucleotides from dephosphorylation and slower the degradation down. Oligonucleotides protected from 3'- or 5'-ends are more stable to mycoplasma nucleases.
Full text: (PDF, in Russian)

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