Biopolym. Cell. 1991; 7(5):15-22.
http://dx.doi.org/10.7124/bc.0002EC
Structure and Function of Biopolymers
1D- and 2D-1H NMR investigation of self-association of deoxytetraribonucleoside triphosphates of different base sequence in aqueous solution
1Veselkov A. N., 2Djimant L. N., 1Davies D., 2Parkes H., 2Shipp D.
  1. Sevastopol Instrument Engineering Institute
    33, Universitetskaya Str., Sevastopol, Ukraine, 99053
  2. Birkbeck, University of London
    Malet Str., Bloomsbury, London WC1E 7HX, UK

Abstract

Equilibrium behaviour of self-complementary deoxytetranucleoside triphosphates 5'-d(GpCpGpC), 5'-d(CpGpCpG), 5'-d(ApGpCpT) and 5'-d(ApCpGpT) in aqueous solution have been studied by the method of PMR spectroscopy (500 MHz). Assignments of the tetranucleotides protons have been obtained from both two-dimensional homo-nuclear COSY and NOESY experiments. Concentration and temperature dependences of proton chemical shifts have been investigated. Equilibrium association constants and thermodynamical parameters of molecules self-association have been calculated using theoretical models and regression equations based on experimental results.
Full text: (PDF, in Russian)

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