Biopolym. Cell. 1991; 7(4):97-103.
http://dx.doi.org/10.7124/bc.0002E9
Cell Biology
Chloroplast DNA topoisomerase I from pea leaves
1Sitailo L. A.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Chloroplasts of pea leaves were isolated by step gradient isoosmotic percoll, chloroplast DNA-topoisomerase. was isolated from them by fractionation in two-phase system ammonium sulfate/polyethylenglycol-6000 with the following purification by using column chromatography. The some properties of DNA-topoisomerase was searched. The dependence of relax activity of the enzyme from Mg2+ ions, its blocking by ethydium bromide, the absence of relax activity stimulation by polyamines: spermidine, spermin, cadaverine and the character of distribution of the topoisomers in the gels allow us to characterise the topoisomerase as prokaryotic type I.
Full text: (PDF, in Russian)

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