pYAC-GN, a yeast artificial chromosome vector which codes GUS and APH(3\')II reporter genes for plant cells

Klimyuk, V. I.

doi:10.7124/bc.0002E8

Biopolym. Cell. 1991; 7(4):95-97.

http://dx.doi.org/10.7124/bc.0002E8

Cell Biology

pYAC-GN, a yeast artificial chromosome vector which codes GUS and APH(3')II reporter genes for plant cells

1Klimyuk V. I.

Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

The vector pYAC-GN has been constructed in order to be able to introduce YAC clones into plant cells for functional analysis of large fragments of DNA. To facilitate the selection of plant cells which have taken up YACs, the pYAC-4 was modified by insertion of GUS and APH(3')II reporter genes under 35S promoter of CaMV into the Clal and Sail sites respectively. The polylinker contaning NotI, Clal and Sail unique restriction sites was also introduced into EcoRI site of SUP4.

Full text: (PDF, in Russian)

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