Isolation of asymmetric somatic hybrids of medicago species

Kuchuk, N. V.; Shakhovski, A. M.; Voronin, V. V.; Gleba, Yu. Yu.

doi:10.7124/bc.0002E1

Biopolym. Cell. 1991; 7(4):54-60.

http://dx.doi.org/10.7124/bc.0002E1

Cell Biology

Isolation of asymmetric somatic hybrids of medicago species

1Kuchuk N. V., 1Shakhovski A. M., 1Voronin V. V., 1Gleba Yu. Yu.

Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

Interspecific somatic hybrids of Medicago have been obtained by «gamma-fusion». Kanamycin resistant plants of M. varia and kanamycin resistant callus line of M. lupulina were used as donors. M. sativa and M. borialis were used as recipients. After fusion of gamma-irradiated (300 Gy) mesophyll protoplasts of M. varia with those of M. sativa, 13 kanamycin resistant hybrid clones were selected. We could induce the formation plant regenerants from one of the clones. Chromosome number of regenerated plants was

Full text: (PDF, in Russian)

References

[1] Teoule E, Daffee Y. Recherche d'une méthode fiable de culture de protoplastes, d'hybridation somatique et de régénération chez Medicago. Agronomie. 1987; 7(8):575-84.

[2] D’Hont A, Quetier F, Teoule E, Dattee Y. Mitochondrial and chloroplast DNA analysis of interspecific somatic hybrids of a leguminosae: Medicago (alfalfa). Plant Sci. 1987;53(3):237–42.

[3] Gilmour DM, Davey MR, Cocking EC. Isolation and culture of heterokaryons following fusion of protoplasts from sexually compatible and sexually incompatible Medicago species. Plant Sci. 1987;53(3):263–70.

[4] Damiani F, Pezzotti M, Arcioni S. Electric Field Mediated Fusion of Protoplasts of Medicago sativa L. and Medicago arborea L. J Plant Physiol. 1988;132(4):474–9.

[5] Deak M, Donn G, Feher A, Dudits D. Dominant expression of a gene amplification-related herbicide resistance in medicago cell hybrids. Plant Cell Rep. 1988;7(3):158-61.

[6] Gilmour DM, Davey MR, Cocking EC. Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis. Plant Cell Rep. 1989;8(1):29–32.

[7] Deak M, Kiss GB, Koncz C, Dudits D. Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep. 1986;5(2):97-100.

[8] Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant. 1962;15(3):473–97.

[9] Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50(1):151-8.

[10] An G, Watson BD, Stachel S, Gordon MP, Nester EW. New cloning vehicles for transformation of higher plants. EMBO J. 1985;4(2):277-84.

[11] Ondrrej M, Eder J, Hrouda M, Machackova I, Vlasak J. Free auxin level and inheritance of introduced markers in tobacco transformed by binary vector eased on A4 Ri plasmid. Biol Plant. 1989;31(4):286–91.

[12] Kuchuk NV. Isolation and cultivation of protoplasts from five legume species. Fiziologiia rasteniy. 1989; 36(4):821-4.

[13] Negrutiu I, De Brouwer D, Watts JW, Sidorov VI, Dirks R, Jacobs M. Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia, Viviani. Theor Appl Genet. 1986;72(2):279-86.

[14] Schreier PH, Seftor EA, Schell J, Bohnert HJ. The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J. 1985;4(1):25-32.

[15] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.

[16] Dellaporta SL, Wood J, Hicks JB. A plant DNA minipreparation: Version II. Plant Mol Biol Rep. 1983;1(4):19–21.

[17] Wijbrandi J, Vos JGM, Koornneef M. Transfer of regeneration capacity from Lycopersicon peruvianum to L. esculentum by protoplast fusion. Plant Cell Tiss Organ Cult. 1988;12(2):193–6.

[18] Famelaer I, Yu Gleba Y, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, et al. Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by “gamma-fusion.” Plant Science. 1989;61(1):105–17.

[19] Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by "gamma-fusion". Theor Appl Genet. 1988;76(5):760-6.

[20] Borisjuk NV, Momot VP, Gleba Y. Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa. Theor Appl Genet. 1988;76(1):108-12.